repartition glucose partially away from glycolysis

HPLC analysis indicated the DG extract contained the fol lowing danshensu, sign ingredients, salvianolic acid W, protocatechuic aldehyde, puerarin, daidzein 8C apiosyl glucoside, daidzin and daidzein. Pharmacokinetics reports indicated that only danshensu, puerarin and daidzein were detectable in plasmat 30 min after oral administration of DG extract to rats at dose fasudil ic50 of 0. 15 gkg. All experimental procedures were approved by the Investigation Practice Committee at the HKUST. Animals received an intraperitoneal injec tion of ISO at of 200 mgkg for the induction myocardial damage. Pre liminary studies indicated that the ISO government increased plasmenzyme actions within six hours in the subjects. Get a grip on animals received the vehicle only. Blood samples were obtained from phenobarbital anesthetized rats at increasing time Mitochondrion intervals post ISO management. These rats were then sacrificed by excision. Myocardial ventricular tissue samples were obtained for the planning of cytosolic and mitochondrial fractions for bio-chemical analyses. Basal values of myocardial mitochondrial parameters and plasmenzyme actions were obtained from animals sacrificed immediately after the injection of saline. DG post-treatment protocol Animals were intragastrically used using the DG extract at amount of 4 gkg soon after intraperito neal shot of ISO within the rat model of ISO induced acute myocardial injury. Initial studies indicated that oral administration of the DG extract at 2 gkg did not produce any detectable changes in plasmenzyme activities four hours after intraperitoneal injection of ISO in mice. Inhibitors of PKC and mKATP PKC translocation chemical and 5 hydroxydecanoate, which are inhibi tors of mKATP and PKC respectively, were TIC10 ic50 dissolved in DMSO at focus of 400 ugmL. Mice were injected using the inhibitor at 400 ug per kilogram of body weight for one-hour before the intragastric administration of DG extract or car. Control animals received 1. 64-40 DMSO in saline. Preparation of plasmsamples and myocardial mitochondrialcytosolic fragments Blood was drawn from phenobarbital anesthetized rats by cardiac puncture in to syringe rinsed with 52-42 Na2EDTas anti coagulant. The blood john ple was centrifuged at 600 g for 10 min at 4 C. The superntants were collected as plasmsamples. Myocardial ventricular tissue samples were washed with ice cold isotonic buffer.